Covalent attachment of DNA oligonucleotides to glass
G Cohen, J Deutsch, J
Fineberg and A Levine
Racah Institute of Physics, Hebrew University of Jerusalem School of Pharmacy, Israel.
A method for
synthesizing DNA directly on glass is presented. The process is
based on pretreatment of glass with boiling hydrochloric acid, thus exposing
the hydroxyl groups of the glass. Phosphite- triester chemistry was used to directly attach
the first nucleotide to a glass plate via a covalent bond between
the hydroxyl group of the glass and the phosphate group of the
protected deoxyribonucleotide. Standard molecular
biology procedures, such as ligation and
restriction digest, were efficiently performed on the glass
synthesized oligonucleotide, with the added benefit
of extreme ease of handling.